Table 4.
Real Time PCR amplification of mGluR5, NMDA2B and GLAST mRNA in the Cerebral cortex of experimental rats
| Animal Status | Log RQ | ||
|---|---|---|---|
| mGluR5 | NMDA2B | GLAST | |
| Control | 0 | 0 | 0 |
| 6-OHDA | 3.55 ± 0.24a | 2.14 ± 0.12a | -2.03 ± 0.11a |
| 6-OHDA +5HT | 2.56 ± 0.12b, f | 1.65 ± 0.22b, f | -1.71 ± 0.14b, f |
| 6-OHDA +GABA | 2.64 ± 0.22b, f | 1.68 ± 0.19b, f | -1.81 ± 0.08b,f |
| 6-OHDA +BMC | 3.41 ± 0.24a | 2.10 ± 0.18a | -2.00 ± 0.06a |
| 6-OHDA +5HT + BMC | 1.52 ± 0.29c, e | 0.89 ± 0.15c, e | -1.11 ± 0.19c, e |
| 6-OHDA + GABA + BMC | 1.84 ± 0.19c, e | 0.92 ± 0.18c, e | -1.13 ± 0.12c, e |
| 6-OHDA +5HT + GABA+ BMC | 0.81 ± 0.10d | 0.41 ± 0.12d | -0.32 ± 0.12d |
Values are Mean ± S.E.M of 4-6 separate experiments. Each group consist 6-8 rats.
a p < 0.001, b p < 0.01, c P < 0.05 when compared to Control,
d p < 0.001, e p < 0.01, f P < 0.05 when compared to 6-OHDA group.
C - Control, 6-OHDA - 6-OHDA infused, 6-OHDA +5-HT - 6-OHDA infused treated with Serotonin, 6-OHDA +GABA - 6-OHDA infused treated with GABA, 6-OHDA +BMC- 6-OHDA infused treated with BMC, 6-OHDA +5-HT+BMC- 6-OHDA infused treated with Serotonin and BMC, 6-OHDA + GABA +BMC- 6-OHDA infused treated with GABA and BMC, 6-OHDA +5-HT + GABA+ BMC- 6-OHDA infused treated with Serotonin, GABA and BMC. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT value as the internal control and Control CT-value as the calibrator.