Table 5.

Real Time PCR amplification of bax, ubiquitin carboxy-terminal hydrolase, α-Synuclien and CREB mRNA in the Cerebral cortex of experimental rats

Animal Status	Log RQ
	bax	ubiquitin carboxy-terminal hydrolase	α-Synuclien	CREB
Control	0	0	0	0
6-OHDA	1.96 ± 0.18a	0.99 ± 0.06 a	-3.12 ± 0.31a	-2.91 ± 0.22a
6-OHDA +5HT	1.02 ± 0.19 b, f	0.51 ± 0.05 b, f	-1.41 ± 0.29b,e	-1.32 ± 0.13b, f
6-OHDA +GABA	1.06 ± 0.11 b, f	0.50 ± 0.07 b, f	-1.55 ± 0.26b,e	-1.43 ± 0.12b, f
6-OHDA +BMC	1.79 ± 0.10a	0.98 ± 0.04 a	-2.99 ± 0.24a	-2.65 ± 0.21a
6-OHDA +5HT + BMC	0.64 ± 0.10 c, e	0.23 ± 0.06c, e	0.12 ± 0.09d	-0.56 ± 0.08c, e
6-OHDA + GABA + BMC	0.61 ± 0.07c,e	0.26 ± 0.04c, e	0.13 ± 0.12d	-0.59 ± 0.09c, e
6-OHDA +5HT + GABA+ BMC	0.29 ± 0.06d	0.11 ± 0.02d	0.41 ± 0.13d	0.09 ± 0.03d

Values are Mean ± S.E.M of 4-6 separate experiments. Each group consist 6-8 rats.

^a p < 0.001, ^b p < 0.01, ^c P < 0.05 when compared to Control,

^d p < 0.001, ^e p < 0.01, ^f P < 0.05 when compared to 6-OHDA group.

C - Control, 6-OHDA - 6-OHDA infused, 6-OHDA +5-HT - 6-OHDA infused treated with Serotonin, 6-OHDA +GABA - 6-OHDA infused treated with GABA, 6-OHDA +BMC- 6-OHDA infused treated with BMC, 6-OHDA +5-HT+BMC- 6-OHDA infused treated with Serotonin and BMC, 6-OHDA + GABA +BMC- 6-OHDA infused treated with GABA and BMC, 6-OHDA +5-HT + GABA+ BMC- 6-OHDA infused treated with Serotonin, GABA and BMC. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT value as the internal control and Control CT-value as the calibrator.