Figure 2.

Comparison of microarray experiments performed by hybridization of gDNA and cDNA. The gDNA curve corresponds to the distribution of the Cy3-channel signals from the reference-reference experiment, while the cDNA curve represents the first control sample of a previous gene expression experiment carried out with the same strain and the same array under similar hybridization conditions [15]. In cDNA experiments, many targets are present at low copy number (low signal) and a few sequences are present in high copy number (high signal). In contrast, in gDNA experiments, most targets are present at the same copy number, resulting in a peak at medium intensity.