Figure 5.
PI3K mediates signaling between MAC1 receptor and PHOX. Microglia-enriched cultures from MAC1+/+ mice were pretreated with wortmannin for 30 min and then challenged with Aβ for 10 min. (A) Extracellular superoxide generation was measured by the SOD-inhibitable reduction of tetrazolium salt, WST-1. (B) Western blot assays for p47phox levels in membrane fractions from microglia. (C) Densitometry analysis was performed with values of p47phox normalized to loading control and further normalized to control levels. (D) Microglia-enriched cultures from MAC1+/+ mice were treated with vehicle medium (control group) or different concentrations of wortmannin for 12 or 24 hours; cell viability was measured using MTT assay. Data are presented as mean ± SEM for three independent experiments. #: p < 0.05 relative to vehicle-treated control cultures. *: p < 0.05 relative to Aβ-treated cultures.