Figure 6.

Exine Defects in Pollen Grains of Single pksa-1 or pksb-2 Mutants.

Pollen grains from mature wild-type (Col-0) plants ([A], [D], [G], [J], and [M]) and plants homozygous for the pksa-1 ([B], [E], [H], [K], and [N]) and pksb-2 ([C], [F], [I], [L], and [O]) alleles were analyzed by auramine O staining, scanning electron microscopy, and TEM. Bars = 10 μm in (A) to (I), 2 μm in (J) to (L), and 1 μm in (M) to (O). Arrows in (H) indicate defective exine patterning and in (L) indicate globular protrusions observed in the pksb-2 mutant.

(A) to (C) Auramine O staining of Col-0, pksa-1, and pksb-2 pollen, respectively.

(D) to (L) Scanning electron microscopy images of pollen samples at three magnification values.

(M) to (O) TEM images of Col-0, pksa-1, and pksb-2 pollen grains, respectively.

[See online article for color version of this figure.]