Figure 7.

Phenotypic Characterization of Plants Segregating for pksa and pksb Alleles.

Plants homozygous for pksa-1 and pksb-3 alleles were crossed, and an F1 individual self-pollinated to generate a population segregating for pksa-1 and pksb-3 alleles. Plants were subjected to PCR-aided genotyping and grown to maturity. PKSA alleles are symbolized by A (WT) and a (pksa-1), and PKSB alleles are symbolized by B (WT) and b (pksb-3). Phenotypes of mature aabb, aaBb, and AABB (WT) plants are shown. Double mutant plants (aabb) had no pollen in anthers (black arrow) and undeveloped siliques (arrowheads), resulting in a complete absence of seeds, whereas Col-0 anthers had abundant pollen at this stage (white arrows). However, there are no other obvious morphological differences except that they flowered for a longer time.

[See online article for color version of this figure.]