Figure 11.

Root Defects in nakr1-1 Appeared after Germination.

(A) Expression of quiescent center marker JYB1234 (At5g17800pro:ER-GFP) in Col-0 and nakr1-1 primary roots at 7 DAG. Images were collected by confocal microscopy. Bars = 10 μm.

(B) Starch accumulation pattern in root columella cells of Col-0 and nakr1-1 seedlings at 1 and 4 DAG. The position of columella cell layers and columella stem cells were labeled with arrowheads and arrows, respectively. Note, at 4 DAG, Col-0 roots showed starch staining in four layers of columella cells, while nakr1-1 roots showed starch in only three layers. Bars = 10 μm.

(C) Cyclin:GUS expression in Col-0 and nakr1-1 roots at 3 and 4 DAG. Bars = 40 μm.

(D) Primary root growth of nakr1-1 and Col-0 within the first 2 weeks after germination. Within the first week, 20% of nakr1-1 primary roots stopped growing, and their primary roots were not measured in the second week. Col-0 and nakr1-1 seedlings were grown vertically on ATS plates under long-day conditions (16-h day/8-h night). Experiments were repeated three times under the same conditions. The y axis value at each time point represents mean ± sd.