Figure 7.

nakr1-1 Mutants Are Defective in Phloem Function.

(A) I₂/KI staining of Col-0 and nakr1-1 leaves, sampled at the beginning and end of the day. Col-0 and nakr1-1 plants were grown in the same tray under short-day conditions for 12 weeks. Mature healthy leaves were sampled for I₂/KI staining and starch quantification.

(B) to (E) Autoradiography of individual Col-0 ([B] and [C]) and nakr1-1 ([D] and [E]) plants exposed to ¹⁴C-sucrose. Plants were grown in Turface. One fully expanded rosette leaf was labeled with 2 μL of ¹⁴C-sucrose (indicated by arrows). After 30 min, the application site was rinsed, and plants were dissected, dried overnight at 20°C, and exposed to film for 5 d ([C] and [E]). Arrowhead in (C) indicates appearance of radioactivity in the root of Col-0.