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EXPOs Are Not Affected by Secretory and Endocytosis Inhibitors in Protoplasts.
Arabidopsis protoplasts were coelectroporated with Exo70E2-(X)FP and marker organelle DNA as indicated and treated with either BFA, wortmannin (1 h), or ConcA (13 h) before observing by CLSM. For inhibition of ER export, protoplasts expressing both Exo70E2-GFP and ManI-mRFP were additionally electroporated with Sec12. DIC, differential interference contrast. Bar = 50 μm.
