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. 2010 Dec 30;22(12):4009–4030. doi: 10.1105/tpc.110.080697

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© 2010 American Society of Plant Biologists

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Figure 5. — Confocal Characterization of Transgenic Arabidopsis or Tobacco BY-2 Cell Lines Expressing Exo70E2-GFP and Exo70E2.

(A) The expression patterns of Exo70E2-GFP in both cell lines are similar to that seen by transient expression in protoplasts. DIC, differential interference contrast.

(B) At Exo70E2a/b antibodies specifically recognize the Exo70E2-GFP signals in both transgenic Arabidopsis and BY-2 cell lines.

(C) At Exo70E2a/b antibodies recognize the endogenous At Ex070E2 proteins in wild-type Arabidopsis cells with similar patterns as transgenic cells expressing Exo70E2-GFP. Bar = 50 μm.