Confocal Characterization of Transgenic Arabidopsis or Tobacco BY-2 Cell Lines Expressing Exo70E2-GFP and Exo70E2.
(A) The expression patterns of Exo70E2-GFP in both cell lines are similar to that seen by transient expression in protoplasts. DIC, differential interference contrast.
(B) At Exo70E2a/b antibodies specifically recognize the Exo70E2-GFP signals in both transgenic Arabidopsis and BY-2 cell lines.
(C) At Exo70E2a/b antibodies recognize the endogenous At Ex070E2 proteins in wild-type Arabidopsis cells with similar patterns as transgenic cells expressing Exo70E2-GFP. Bar = 50 μm.