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. 2010 Dec 17;22(12):4176–4194. doi: 10.1105/tpc.110.077461

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Figure 1. — Rx Localizes to the Cytoplasm and Nucleus in Transgenic N. benthamiana:Rx4HA.

Immunoblot showing the presence of Rx-4HA in nuclei-depleted soluble (Cyto) and nuclear (Nuc) fractions of transgenic N. benthamiana:Rx4HA plants, which express Rx-4HA under control of the Rx native regulatory sequences. Fractionation was performed in four replicates. The nuclei-enriched fraction was loaded in a relatively 10-fold higher concentration. Hsp90, SGT1, and histone H3, detected by immunoblotting, were used as fractionation markers. Coomassie blue (CBB) staining of the blot was used as loading control.