Figure 7.

Hyperaccumulation of Rx in the Nucleus Blocks Its Auto-Activity.

(A) Confocal images of N. benthamiana leaf epidermal cells transiently coexpressing GFP-Rx or GFP-Rx-CC-NB (detected by the GFP channel) with the indicated RanGAP2 (Rg2) constructs fused to mC, or mC alone (detected by the mCherry channel). Red structures are chloroplasts. C, cytoplasm; N, nucleus. Bars = 10 μm.

(B) The weak and strong auto-active Rx variants Rx-CC-NB-HA and Rx(D460V), respectively, were transiently coexpressed in N. benthamiana (at OD₆₀₀ = 0.3 and 0.1, respectively) with the indicated Rg2 constructs or GUS in circular areas. Chlorophyll was removed to visualize the HR, which appears as a dark spot. The subcellular localization of Rx is indicated below the pictures.