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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Can J Physiol Pharmacol. 2008 Jun;86(6):310–319. doi: 10.1139/y08-025

Figure 1.

Figure 1

Expression of mRNA for Edn2 in the mouse ovary during gonadotropin-primed follicular development and ovulation. Immature mice were treated with 5 IU PMSG to induce follicular development and with 5 IU hCG 48 h later to synchronize ovulation. A) Animals were sacrificed for tissue collection at 48 h after PMSG and at 6, 12 and 24 h after hCG (n = 3 per time point). Real-time PCR was performed to determine expression of mRNA for Edn2. Expression of mRNA for GAPDH was used to normalize data. Expression of mRNA for Edn2 was increased at 12 h after hCG in comparison to all other time points examined (p < 0.05). B) Animals were sacrificed for tissue collection at 48 h after PMSG and at 12 h after hCG (n = 2 per time point). Northern analysis was used to determine expression and transcript size of mRNA for Edn2. Expression of 18S rRNA was used as a loading control.