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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Can J Physiol Pharmacol. 2008 Jun;86(6):310–319. doi: 10.1139/y08-025

Figure 2.

Figure 2

Induction of mRNA for Edn2 by hCG in vivo vs. in vitro. Real-time PCR analysis of the induction of mRNA for Edn2 in ovaries of PMSG/hCG-primed mice sacrificed at 12 h after treatment with hCG (in vivo control) and in granulosa cells collected from PMSG-primed mice sacrificed at 48 h after treatment with PMSG and cultured for 0, 6 and 12 h with 0 or 5 IU hCG. Expression of mRNA for Edn2 was increased in hCG-treated granulosa cells after 12 h in culture (p < 0.05). However expression of mRNA for Edn2 after treatment of granulosa cells with hCG for 12 h in vitro was low compared to the in vivo control.