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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Can J Physiol Pharmacol. 2008 Jun;86(6):310–319. doi: 10.1139/y08-025

Figure 4.

Figure 4

Effect of PR or COX-2 on Edn2 expression. A) Ovulation rate in response to treatment with vehicle, RU-486 or indomethacin. Immature mice were treated with PMSG and 48 h later with hCG. Vehicle (DMSO), RU-486 (10 mg/kg body weight) or indomethacin (10 mg/kg body weight) were administered at PMSG + 48 h, 47 h and 49 h, respectively. One ovary per mouse was collected at 12 h after hCG for RNA extraction. The rate of ovulation for the contralateral ovary was determined at 20 h after hCG. Filled circles represent individual mice. Ovaries collected at 12 h after hCG from the mice represented by filled circles enclosed in boxes were utilized for the determination of Edn2 mRNA expression. B) Real-time PCR analysis of Edn2 mRNA expression, relative to GAPDH. No effect of treatment with vehicle, RU-486 or indomethacin on the expression of mRNA for Edn2 was detected (p > 0.05).