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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Can J Physiol Pharmacol. 2008 Jun;86(6):310–319. doi: 10.1139/y08-025

Figure 5.

Figure 5

Analysis of Edn2 mRNA by RACE PCR and the nucleotide sequence of the EDN2 proximal promoter region. To determine the transcription initiation site (TIS), 5′ RACE PCR was carried out from total RNA extracted from an ovary at 12 after hCG. The PCR products were separated on agarose gel. A) The main PCR product is indicated by an arrow (Lane 1, 100 bp marker; lane 2, RACE PCR products). B) The nucleotide sequence of the EDN2 promoter region and part of exon 1. The TIS is bolded and assigned by the number +1. The translational initiation codon is boxed. Possible regulatory elements and binding factor were searched on TESS program. Elements with a likelihood value of 20 or greater are underlined with the names of their possible interacting factors.