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Effects of deglycosylation by PNG-aseF on abdominal vitellogenin on SDS-PAGE. S, molecular weight standard. (A) Deglycosylation of the 40 kDa vitellogenin. The deglycosylated sample appears as a ∼2.5 kDa lighter molecular weight band. PNGaseF, the deglycosylation enzyme. (B) Deglycosylation of the 180 and 150 kDa vitellogenin. The full-length vitellogenin shifts ∼4.2 kDa and the 150 kDa vitellogenin shifts ∼2.4 kDa after the removal of N-linked glycans.
