Fig. 5.

Validation of AamS6 protein secretion into tick saliva. (A) Pilocarpine-induced tick saliva harvested from ticks fed for the indicated time periods was subjected to western blot analysis using antibodies to rAamS6 or pre-immune sera as indicated. (B) Affinity-purified rAamS6 was subjected to western blot analysis using antibodies to tick saliva proteins raised by repeated 48 h infestations of rabbits with adult A. americanum or pre-immune sera as indicated. In B, lane 1 is affinity-purified rAamS6, lane 2 is a 10-fold dilution of lane 1, lanes 3 and 4 are protein extracts of unfed and 24 h partially fed ticks, respectively.