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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Tissue Antigens. 2009 Apr;73(4):335–340. doi: 10.1111/j.1399-0039.2009.01219.x

Table 1.

Oligonucleotide sequences for HLA typing of DQB1*0602.

Oligo Name Description Sequence (5′ to 3′) Final concentration (nM)
DQB1 typing:
GDQ1 DQB forward 132 primer GGGCATGTGCTACTTCACCAA 225
GDQ2 DQB reverse 209 primer GCGTACTCCTCTCGGTTATAGATGT 225
GDQ3 DQB 167 probe 1 FAM-TGCGTCTTGTGACCAG-MGB 50
GDQ4 DQB 167 probe 2 VIC-TGCGTCTTGTAACCAG-MGB 50
GDQ5 DQB forward 155 primer GGACGGAGCGCGTGCGTCTT 225
GDQ6 DQB reverse 294 primer TTCCTTCTGGCTGTTCCAGTACT 225
GDQ7 DQB 245 probe 1 FAM-ACCGCGCGGTACA-MGB 50
GDQ8 DQB 246 probe 2 VIC-CACCGCCCGATAC-MGB 50
DRA control:
GDR1 DRA forward primer AGGCCGAGTTCTATCTGAATCCT 225
GDR2 DRA reverse primer CGCCAGACCGTCTCCTTCT 225
GDR3 DRA probe VIC-CATAAACTCGCCTGATTG-MGB 50