Table 1.
Oligo Name | Description | Sequence (5′ to 3′) | Final concentration (nM) |
---|---|---|---|
DQB1 typing: | |||
GDQ1 | DQB forward 132 primer | GGGCATGTGCTACTTCACCAA | 225 |
GDQ2 | DQB reverse 209 primer | GCGTACTCCTCTCGGTTATAGATGT | 225 |
GDQ3 | DQB 167 probe 1 | FAM-TGCGTCTTGTGACCAG-MGB | 50 |
GDQ4 | DQB 167 probe 2 | VIC-TGCGTCTTGTAACCAG-MGB | 50 |
GDQ5 | DQB forward 155 primer | GGACGGAGCGCGTGCGTCTT | 225 |
GDQ6 | DQB reverse 294 primer | TTCCTTCTGGCTGTTCCAGTACT | 225 |
GDQ7 | DQB 245 probe 1 | FAM-ACCGCGCGGTACA-MGB | 50 |
GDQ8 | DQB 246 probe 2 | VIC-CACCGCCCGATAC-MGB | 50 |
DRA control: | |||
GDR1 | DRA forward primer | AGGCCGAGTTCTATCTGAATCCT | 225 |
GDR2 | DRA reverse primer | CGCCAGACCGTCTCCTTCT | 225 |
GDR3 | DRA probe | VIC-CATAAACTCGCCTGATTG-MGB | 50 |