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. 2011 Jan 26;6(1):e16074. doi: 10.1371/journal.pone.0016074

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) SDS-PAGE gel of ES proteins used as immunogens after affinity purification. The ES proteins possessing the C-terminal heterologous T cell helper residues are denoted “+TH”. (B) The recognition of the ES proteins by the 2F5 monoclonal antibody was assessed by surface plasmon resonance (SPR) in a Biacore 3000 instrument. In red, the observed data obtained by flowing the ES proteins as analytes over a CM5 chip to which the 2F5 IgG antibody was immobilized. In black, fit curves when a 1∶1 Langmuir model is applied to the observed data. Affinity constant values are indicated above the curves and the rate constants are denoted below the curves.