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. 2011 Jan 26;6(1):e15642. doi: 10.1371/journal.pone.0015642

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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PS and Pr fluorescent expression for Jurkat (A,C) and HeLa (B,D) exposed to increasing electric fields between 0–150 kV/cm at 100 pulses per exposure and 0.005% digitonin. Externalization of PS appears in Jurkat at lower field amplitudes than in HeLa. Both cell lines exposed to digitonin stained positive for both PS and Pr. C and D show percent of Pr positive Jurkat and HeLa after exposure to increasing number of pulses at 217 and 285 kV/cm, respectively. (mean +/− s.d., n = 3 measurements of 25,000 cells).