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. 2011 Jan 26;6(1):e16199. doi: 10.1371/journal.pone.0016199

Figure 4. The interaction between frataxin and the Iscu/Nfs1/Isd11 complex is essential for cellular function.

Figure 4

(A) Western blot analysis of mitochondria-enriched fractions from clones expressing wild type frataxin, mito81–210, FXNN146A and FXNN146K using anti-frataxin and anti-tubulin antibodies. (B) Morphological and ultrastructural alterations in FXN, N146A, N146K and mito81–210 clones. Each clone was studied by phase contrast microscopy after crystal violet staining (left panels) and electron microscopy analysis (right panels). mt, mitochondria; Lp, lipid droplet; mt-Fe, intramitochondrial iron deposits; N, nucleus. (C) Biochemical measurements of Fe-S enzyme activities in FXN, N146A, N146K and mito81–210 clones. Succinate dehydrogenase (grey bars) and aconitases (dark grey bars) specific activities were standardized to isocitrate dehydrogenase (IDH) specific activity and expressed as percentage of control activity. Results were obtained from two independent experiments using 4 FXN, 3 N146A, 1 N146K and 4 mito81–210 clones. Data are represented as mean + SD. * p<0.05.