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. 2011 Jan 26;6(1):e16245. doi: 10.1371/journal.pone.0016245

Figure 2. Direct comparison of conventional enzymatic methods and lung matrix explant methods.

Figure 2

T cells were extracted by conventional method and explant method. PBMCs were isolated by Ficoll-hypaque density gradient method. Cells were stained for surface markers and analyzed by flow cytometry. (A) Expression of CD45RO (TEM) and CD45RA on lung-CD3+T cells and blood-T cells was analyzed. (B–E) Many CD4 and CD8+T cells express CD69, HLA-DR and only few CD4+T cells but not CD8 express CD25. (F) Most of the lung-T cells are αβ+ and very few express γδ TCR. (G) A subpopulation of CD4+ CD45RA- T cells also express L-selectin and CCR7 (markers for central memory phenotype). Dot plots are representative of 9 experiments (3A–F) that produced similar results.