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. 2011 Jan 26;6(1):e16503. doi: 10.1371/journal.pone.0016503

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Castillo-Olivares et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Livers of normal uninfected (a) and AHSV-4 infected (b) mice were processed for immuno-fluorescence as indicated in Materials and Methods. The presence of AHSV antigen in the liver (green fluorescence) accumulates in foci of cells that resemble the foci of necrosis observed in hematoxylin-eosin sections.