The effect of sarafotoxin S6c (1 µM) was analyzed with or without a 10-minute pre-incubation period with either 1 µM BQ-123, BQ-788, nifedipine or 10 mM EGTA, and compared to the contraction induced by ET-1 alone (0.1 µM). (A) Representative recordings of isometric tension changes illustrating the effect of sarafotoxin S6c in presence (right panel) or in absence (left panel) of BQ-123 (other drugs not shown). (B) Average contraction obtained with sarafotoxin S6c under basal conditions (CTL) or in the presence of BQ-123, BQ-788, EGTA or nifedipine. Data, expressed as a percentage of the contraction induced by ET-1 (0.1 µM) in the same experiment, indicate the means ± S.E.M. of 10 different arteries, with experiments performed in triplicate. *** p<0.0001.