Fig. 6.

T-cells recovered from immunized huPBL-NSG mice respond to antigen-specific stimulation in vitro. Spleen cells recovered from huPBL-NSG mice that were previously immunized with DC/AdV-GFP in vivo were labeled with CFSE and co-cultured with either control DC or DC/AdV-RR5 (DC/T ratio = 1:20) for 5 days in vitro. a Cells were stained with anti-CD3 and anti-CD4 and the CD3+ T-cells analyzed for adenoviral-specific proliferative responses by flow cytometry. Representative experiment, n = 5. b The sub-population of cells proliferating in response to stimulation with DC/AdV-RR5 (CFSE-dim) were further analyzed for their expression of effector/memory and regulatory T-cell markers. c Supernatants from the same wells were harvested and assessed for the presence of human IFN-γ and TNF-α by SearchLight multiplex assay. Representative experiment, n = 3. *p < 0.01