Figure 7.

Lung IDO expression in CC10-hTLR9 mice exposed to CpG DNA. (A) Tissues from CC10-hTLR9 transgenic mice on a TLR9-deficient background were analyzed by RT-PCR for lung-specific human Toll-Like-Receptor 9 (hTLR9) transgene expression. Transgene-negative (Tg⁻) and CC10-hTLR9 transgenic (CC10-hTLR9 Tg⁺) mice were administered 10 μg CpG DNA into their airways by oropharyngeal aspiration. Twenty-four hours later, pulverized lungs were analyzed by quantitative RT-PCR for relative expression levels of hTLR9 transgene (B), mouse TLR9 (mTLR9) (C), CCL20 (D), and IDO (E). Values are means (± SEM) of five mice per group, and are representative of experiments performed twice.