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. 2011 Jan 21;60(2):436–442. doi: 10.2337/db10-0352

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© 2011 by the American Diabetes Association.

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FIG. 3. — Glucoprivic feeding and AMPK expression in DSAP- or SAP-injected rats. A: Food intake during the 4-h period after 2DG (200 mg/kg s.c.) or saline (Sal) injection in SAP and DSAP rats. *P < 0.001 vs. SAP rats with saline injection (n = 16 rats per group). B: TH expression levels in A1–C1m regions in SAP and DSAP rats, measured by Western blot. The level of TH in SAP rats, normalized to β-actin, was used as 100%. *P < 0.001 vs. SAP rats (n = 16 rats per group). C: Upper panel: immunoblots of pAMPKα and β-actin in A1–C1m regions 45 min after saline or 2DG injection. Lower panel: quantitative pAMPKα data, normalized to β-actin. *P < 0.05 vs. saline-injected SAP rats; #P < 0.05 vs. 2DG-injected SAP rats. Fisher PLSD test after one-way ANOVA (n = eight rats per group). D: Upper panel: immunoblots of total AMPKα and β-actin in A1–C1m regions 45 min after saline or 2DG injection. Lower panel: quantitative data, normalized to β-actin (n = eight rats per group).