FIG. 4.

PI4P5Kα KO islets secrete insulin with altered kinetics on glucose stimulation. WT and KO islets (10 islets per experimental sample, experiment performed six times) were isolated from age- and sex-matched mice, preincubated in nonstimulatory culture media (2.8 mmol/L glucose), and challenged with 20 mmol/L glucose. Six mice were used for each genotype. Incubation times were 30 min in succession for each condition. Stimulated insulin release is presented after subtraction of the basal release. Data are presented as mean ± SEM.