FIG. 3.

The aP2-dn-JNK mice were less obese than control mice under the HFD, displaying decreased fat mass and triglyceride contents in adipose tissue. A: Body weight changes on standard chow (SC) or HFD (HF) over 24 weeks in aP2-dn-JNK transgenic (Tg) mice and their wild-type (WT) littermates. B: Caloric intake, as calculated by food intake per mouse per week (g/mouse/week) × 3.07 kcal/g on the standard chow and × 4.73 kcal/g on the HFD. C: Fat-pad weights in aP2-dn-JNK and wild-type littermates, as measured after dissection at the age of 24 weeks and presented as percentage of total body weight. Insert: Adiposity index, calculated as the total weight of fat pads divided by total body weight. Fat pads: BAT, subcutaneous (SU), epididymal (EPI), perirenal (PRF), interscapular (Int), WAT, and gluteal fat (Glu). D: Representative images of hematoxylin and eosin–stained sections of epididymal WAT and interscapular BAT on the HFD at the age of 24 weeks. Scale bar: 50 μm. E: The size of adipocytes quantified using NIH ImageJ software. F: Triglyceride contents in epididymal fat pads and brown fat pads. *P < 0.05; **P < 0.01 vs. wild-type littermates (n = 6–8). A and B were analyzed by ANOVA; C, E, and F were analyzed by the Student t test. (A high-quality color representation of this figure is available in the online issue.)