TABLE 2.
Nucleosome loss is greatest in highly transcribed genesa
| Gene | Nucleosome level in spt6-1004 | Spt6 level | RNAP II level |
|---|---|---|---|
| PGK1 | −1.03 | 2.19 | 4.74 |
| CHA1 | −1.00 | 1.07 | 2.24 |
| PMP1 | −0.89 | 1.67 | 3.57 |
| PDI1 | −0.79 | 1.23 | 2.58 |
| RPS14A | −0.78 | 1.81 | 3.15 |
| GLK1 | −1.36 | 0.76 | 1.96 |
| VAC17 | 0.01 | 0.41 | 0.08 |
| BUD3 | −0.02 | −0.22 | −1.11 |
| DCC1 | −0.14 | −0.34 | −0.33 |
| CDC39 | 0 | 0.32 | −0.37 |
| SNT1 | 0.03 | 0.08 | −0.65 |
The RNAP II occupancy (log2 IP/no Ab) and Spt6 occupancy (log2 HA/no tag) determined by our ChIP-chip assay are compared to the nucleosome occupancy signals (log2 spt6-1004/wild type) across coding regions for representative genes on chromosome III. The genes in boldface in the top half of the table are highly transcribed; those in the bottom half are transcribed at basal levels.