FIG. 2.

Thermotolerance of SRSF10 dephosphorylation requires synthesis of heat shock proteins. (A) Thermotolerance of SRSF10 dephosphorylation requires de novo protein synthesis. HeLa cells that were untreated (lanes 1 to 3) or treated with a mild heat shock (lanes 4 to 9) in the absence (lanes 4 to 6) or presence (lanes 7 to 9) of cycloheximide were incubated at 44°C for the indicated times. SRSF10, Hsp70, Hsc70, and Hsp27 were monitored by Western blotting. (B) HeLa cells transfected with control or HSF1 RNAi constructs were left untreated or treated with a mild heat shock and then incubated at 44°C for 0 or 40 min. SRSF10 and HSF1 were monitored by Western blotting. The arrows indicate the faster- (a) and slower-migrating (b) forms of HSF1.