FIG. 1.

pot1Δ rqh1-hd double mutant is viable but is sensitive to TBZ. (A) Spotting assay of 10-fold serial dilutions of cells. The plasmid is retained on Edinburgh minimal medium (EMM) plus adenine and leucine and selected against on YEA plus FUDR at 30°C. The sensitivities to temperature (B) and TBZ (C) of wild-type, rqh1-hd, pot1Δ, and 2 independent pot1Δ rqh1-hd cells were determined using a spot test. The 10-fold dilutions of log-phase cells were spotted onto a YEA plate at the indicated temperature or a YEA plate containing 12.5 μg/ml TBZ at 30°C.