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. 2010 Nov 29;31(3):388–403. doi: 10.1128/MCB.00969-10

FIG. 5.

FIG. 5.

Effect of dominant negative (DN) PAK3 and PAK2 on neuronal complexity. (A) Cultured hippocampal neurons prepared from PAK1 KO pups infected with viruses expressing GFP or DN GFP-PAK3 showing reduced dendritic arbors in DN PAK3 virus-infected but not GFP PAK3 virus-infected neurons. (B) Summary graph showing reduction in neuronal TDBTN in DN PAK3 virus-infected neurons compared to the noninfected control or GFP virus-infected neurons (n = 20 neurons for each treatment). (C) Cultured hippocampal neuron prepared from PAK1 KO pups cotransfected with DN PAK2 (K278R) and GFP (green) plasmid DNA, showing dendritic arbors similar to that of untransfected neuron (blue; MAP2). (D) Summary graph showing no differences in neuronal TDBTN between DN PAK2-transfected and untransfected neurons. Bar, 20 μm.