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. 2010 Nov 29;31(3):388–403. doi: 10.1128/MCB.00969-10

FIG. 6.

FIG. 6.

Reduced axonal growth in PAK1/PAK3 DK mice. (A) Brain sections stained for myelin showing thinner white matter tracts in the DK mice. Scale bar, 2 mm. (B) Hippocampal sections stained for the axon-specific protein phospho-neurofilament (pNF) revealing reduced pNF fluorescence intensity in the CA1 area (arrow) in the DK mice. Scale bar, 300 μm. On the right are enlarged CA1 dendritic areas revealing reduced axonal fragments in the DK section. Scale bar, 50 μm. (C) Hippocampal sections stained for the presynaptic marker synapsin (syn). Scale bar, 300 μm. (D) Summary data showing reduced fluorescence intensity of myelin and pNF but not of synapsin in the DK mice (n = number of brain sections). (E) Cultured hippocampal neurons stained at DIVs 3, 7, and 21 for pNF (green) and F-actin (red) showing reduced axonal growth in DK neurons. Scale bars, 50 μm. (F to H) Summary data showing reduction in both total axon length (F) and branching points (G) but not in average branch length (H) in the DK neurons (n = number of neurons).