Skip to main content
. 2010 Dec 6;31(4):616–625. doi: 10.1128/MCB.00849-10

FIG. 2.

FIG. 2.

Fluorescent tagging of Mcp and Fab-7 components. (A) Structure of the reporter constructs. The Mcp insulator fragment is flanked by LoxP sites, and the Mcp PRE is flanked by FRT sites. A parallel construct contains a similar arrangement of the Fab-7 insulator and the Fab-7 PRE. The constructs utilize the yellow and mini-white genes as markers. The tandem array of 124 lacO sequences is used to bind the lacI repressor fused to EGFP expressed from a different construct driven by the ubiquitin promoter. (B) Mcp and Fab-7 lines obtained and their insertion sites determined by inverse PCR. (C) Representative image of eye imaginal disc nuclei showing one-dot (arrows) and two-dot (arrowheads) nuclei from the F9 F9ΔP-M31ΔP line (ΔP, deletion of PRE). (D). Image of eye membrane cell nuclei showing the two-dot (arrowhead) nuclei from F9ΔI-M31ΔI line (ΔI, deletion of insulator). The images show a single z-axis slice, while the score was obtained by examining all z-axis slices individually.