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. 2010 Dec 13;31(4):674–685. doi: 10.1128/MCB.01188-10

FIG. 8.

FIG. 8.

Genetic analysis of the Thp3 protein complex. (A) Growth on SC-ura of hpr1Δ thp3Δ (BWMH-8D), hpr1Δ csn12Δ (BWCH-1B), hpr1Δ sem1Δ (WSH-2A), and hpr1Δ csn9Δ (BWC9H-4A) mutant congenic strains carrying the pLAUR system and growth of the same strains in YPD at 30 and 37°C. Other details are as in Fig. 1A. (B) Suppression of the hyperrecombination phenotype of the hpr1Δ mutant by the thp3 and csn12 mutations. The recombination frequencies of the plasmid-borne direct-repeat system LYΔNS in the hpr1Δ (BWMN-2A), hpr1Δ thp3Δ (BWMN-3B), and hpr1Δ csn12Δ (WCSHP-3B) mutant congenic strains are shown. A schematic of the recombination assay is shown at the top. (C) Serial dilutions of the wild-type (WT; BY4741) and thp3Δ, csn12Δ, and csn9Δ mutant isogenic strains cultured in YPD medium with and without MPA (150 μg/ml) at 37°C.