FIG. 5.

INO80 cannot mobilize dinucleosomes with only 6 or 30 bp of linker DNA, but it can with 79 bp. (A to C) Dinucleosomal substrates containing 6 (A), 30 (B), or 79 bp (C) of linker DNA between the two nucleosomes were remodeled with INO80. All three nucleosomes contained 9 and 30 bp of flanking DNA on either side. Dinucleosome positions were mapped as for Fig. 2, before and after INO80 remodeling, and are displayed in the same way. On the right side is the schematic view of the nucleosomes moved by INO80. The direction of nucleosome movement and the number of bp moved are indicated by arrows and numbers, respectively. The thickness of the arrow indicates the relative amount of nucleosomes moved. In panel C, the scale on the left side has been expanded to allow better visualization of the location of the remodeled nucleosome positions.