FIG. 9.

INO80 does not require any of the histone tails for efficiently mobilizing nucleosomes. (A) The location of the NotI restriction endonuclease site is shown in terms of the number of bp from the dyad axis of the 601 nucleosome before and after INO80 remodeling. End-positioned nucleosomes with 70 bp of extranucleosomal DNA (0N70) were used for the experiment. The locations of mobilized nucleosomes were previously determined by site-directed mapping. (B) Nucleosomes (33 nM) with (WT) or without (gAll) all the histone tails were remodeled with INO80 (40 nM) and ATP (800 μM). Samples were incubated with NotI and deproteinized, and DNA was analyzed on a 6% native PAGE gel. (C) The nucleosomes were mobilized by INO80 for different lengths of time ranging from 5 s to 21 min and analyzed as described for panel B.