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. 2010 Dec 6;31(4):721–735. doi: 10.1128/MCB.00403-10

FIG. 6.

FIG. 6.

Cbk1 activation requires activation of MEN. (A) Cbk1 exhibits dephosphorylation upon release from MEN arrest. The cdc15-2 strain was released from arrest, and cells were harvested every 15 min. Immunoprecipitated Cbk1 was probed with anti-Cbk1 (top panel) or anti-pT743 (middle panel) antibody. Lambda phosphatase-treated samples are shown on the bottom. (B) Activation of Ace2 target gene expression correlates with Cbk1 dephosphorylation. The cdc15-2 strain was released from arrest, and cells were harvested every 15 min. QPCR was performed to determine the transcript levels CTS1 and DSE1 relative to the levels in arrested cells (0 min). Standard errors of the means from three independent experiments are shown. (C) Strong daughter-specific localization of Ace2 correlates with dephosphorylation of Cbk1. cdc15-2 cells expressing Ace2-GFP were released from arrest, and images were acquired every 2 min. Representative cells were followed for the full time course.