TABLE 2.
RT-qPCR primers
| Target gene | Primer sequence |
Primer position |
NCBI accession no. | ||
|---|---|---|---|---|---|
| 5′ | 3′ | bpa | Exon(s) | ||
| B-actin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC | +336 | 3-4 | NM_007393.3 |
| brachyury | GAAGGCGCCTGTGTCTTTC | CCCCCAACTCTCACGATG | +415 | 2-3 | NM_009309.2 |
| Flk-1 | CCCCATTTCAATGGAGAAAC | TAGGCGAGATCAAGGCTTTC | +1211 | 9-10 | NM_010612.2 |
| Gcn5 | AGAGGAAGGCGCAAGTCC | TCCAGCCATTACACTTGCAG | +250 | 1-2 | NM_001038010.2 |
| Hbo1 | TTTTGGCCGCTATGAACTG | GGAGGATTGTCTGGCTCTTC | +755 | 8 | NM_001195004.1 |
| Hoxa3 | ATGGGACCCCACACTTACAG | GTGTGGGGGAGGTGAGTTAG | +1066 | 5 | NM_010452.3 |
| Hsp90ab1 | ACCTGGGAACCATTGCTAAG | AGAATCCGACACCAAACTGC | +301 | 3-4 | NM_008302.3 |
| Mof | CTCGAAACCAAAAGCGAAAG | CCTCGTGTTCCTTTTCCAAG | +415 | 3-4 | NM_026370.1 |
| Moz | GTCGTGACCAAGGCAAAAAC | GGGTCACAACACTCCATGTG | +802 | 5-6 | NM_001081149.1 |
| Notch1 | TCAGGGTGTCTTCCAGATCC | AGCCAGGATCAGTGGAGTTG | +5888 | 31-32 | NM_008714.3 |
| Otx2 | GAATCCAGGGTGCAGGTATG | TTCTGACCTCCATTCTGCTG | +234 | 2-3 | NM_144841.3 |
| Pgk1 | TACCTGCTGGCTGGATGG | CACAGCCTCGGCATATTTCT | +919 | 8-9 | NM_008828.2 |
| Psmb2 | GAGGGCAGTGGAGCTTCTTA | AGGTGGGCAGATTCAAGATG | +461 | 5-6 | NM_011970.4 |
| Rpl13a | GGAGAAACGGAAGGAAAAGG | TGAGGACCTCTGTGAACTTGC | +470 | 7-8 | NM_009438.4 |
| Sox1 | AACCCCAAGATGCACAACTC | TGTAATCCGGGTGTTCCTTC | +216 | 1 | NM_009233.3 |
| Sox2 | CTCTGCACATGAAGGAGCAC | CCGGGAAGCGTGTACTTATC | +301 | 1 | NM_011443.3 |
| Tbx1 | GTGGACCCTCGAAAAGACAG | GATCCGGTGATTCTGGTAGG | +729 | 4-5 | NM_011532.1 |
| Tie-1 | CCGAAGAAGCTGCCTACATC | TCAAGGTCCCTGAGCTGAAC | +2345 | 14-15 | NM_011587.2 |
| Tie-2 | TCCGAGCTAGAGTCAACACC | CTGGTTGAGGAGGGAGAATG | +1834 | 12-13 | NM_013690.2 |
a
Position on cDNA sequence downstream of ATG.