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. 2010 Dec 20;31(4):686–699. doi: 10.1128/MCB.00019-10

FIG. 1.

FIG. 1.

Upstream elements increase the activity of the Matn1 proximal promoter in vivo. (A) Schematic of PS-NAD1 depicting conserved DNA blocks (rectangles, diamond, and oval) in the distal and proximal Matn1 promoter regions. (B and C) Expression of PS-NAD1 (B) and TR70 (C; 2-kb Matn1 promoter) transgenes in founder embryos (FE) stained with X-Gal at E15.5. (D to L) Histological analysis of PS-NAD1 embryo cryosections. In the developing limbs, X-Gal staining increases proximodistally from humerus (h), ulna (ul), and femur (f) to radius (ra), tibia (ti), and fibula (fi) and further from carpals (c) and metacarpals (mc) to phalanges (ph) (D to H). Staining is absent in cranial (cran) vertebral bodies (vb) but increases from lumbar (lumb) to caudal (caud) regions (I to K). LacZ activity is restricted to the GP zones of columnar chondroblasts (cc) and prehypertrophic chondrocytes (pc), while it is low or absent in the zones of epiphyseal (ec) and source chondroblasts (sc) and hypertrophic chondrocytes (hc) (D, G, and J to L). np, nucleus pulposus; r, rib. Bars, 2 mm (B and C) and 200 μm (D to L).