Skip to main content
. 2010 Dec 10;193(4):822–831. doi: 10.1128/JB.01555-09

FIG. 5.

FIG. 5.

(A) Schematic maps of Sgp2 and amino acid sequences conserved in Sgp2 and pilin and putative pilin subunits of several other Gram-positive bacteria. Sgp2 harbors an N-terminal signal sequence (amino acid positions 1 to 30), a von Willebrand factor type A (vWFA) domain (amino acid positions 468 to 601), a Cna protein B-type (CnaB) domain (amino acid positions 870 to 938), and a CWSS (LPSTG; amino acid positions 963 to 967). Alignment was carried out using the ClustalW program (45). Amino acid sequences conserved in all of the aligned sequences (in uppercase letters) or in more than seven sequences (in lowercase letters) were designated consensus sequences. The GenBank accession numbers of the listed proteins are as follows: ancillary protein 1 of S. pyogenes serotype M6 strains, AAL11466 (MGAS10394), ACH87890 (2724), and ACH87891 (3650); ancillary protein 1 of S. pyogenes M23 strain (DSM 2071), ACH87900; FszD of S. equi subsp. zooepidemicus MGCS10565, ACG63149; CLOSCI_01050 of C. scindens ATCC 35704, EDS08034; BIFDEN_00672 and BIFDEN_01142 of B. dentium ATCC 27678, EDT44861 and EDT45313, respectively; BIFADO_00261 and BIFADO_01716 of B. adolescentis L2-32, EDN83355 and EDN82663, respectively; BbifN4_00972 of B. bifidum NCIMB 41171, ZP_03645701; BIFLAC_00845 of B. animalis subsp. lactis HN019, EDT88620; and HMPREF0628_1483 of P. lacrimalis 315-B, EFA89995. (B) Genetic organizations of the srtG cluster, FCT-1 region, and putative pilus gene clusters containing sgp2 homologues or genes partially homologous to sgp2. Black arrows, genes and putative genes encoding class C sortases; gray arrows, genes and putative genes encoding CWSS-containing proteins (sgp2 and genes encoding proteins partially homologous to Sgp2 are outlined in black); open arrows outlined in gray, putative transcriptional regulator genes; open arrows outlined in black, other open reading frames. These genetic maps are constructed on the basis of the whole or draft genome sequences of the respective strains, and the GenBank accession numbers are as follows: S. pyogenes MGAS10394, CP000003; S. equi subsp. zooepidemicus MGCS10565, CP001129; C. scindens ATCC 35704, ABFY02000010; B. dentium ATCC 27678, ABIX02000002; B. adolescentis L2-32, AAXD02000018 and AAXD02000052; B. bifidum NCIMB 41171, ABQP01000004; B. animalis subsp. lactis HN019, ABOT01000008; and P. lacrimalis 315-B, ADDO01000052.