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Map of Tn′blaTEM-1. Plasmid pJES124 contains the signal sequence-less ′blaTEM-1 gene cloned downstream of the left inverted repeat (LIR) of a Himar1-based mariner transposon derived from pSH1. The codon sequence of the inverted repeat is shown in the same reading frame as the starting codons of ′BlaTEM-1. Tnpmar, mariner transposase; hygR, hygromycin resistance gene.
