FIG. 3.
M. tuberculosis rv0199::Tn′blaTEM-1 has a growth defect in macrophages. (A) Organization of genes surrounding Tn′blaTEM-1 insertion (triangle) in rv0199. (B) Membrane topology of Rv0199 predicted by the TMHMM algorithm (49). The site of ′BlaTEM-1 fusion is indicated with a triangle. (C) Macrophages were infected with MBTB126 carrying the empty vector pMV261.kan, the rv0199::Tn′blaTEM-1 mutant carrying pMV261.kan, or the rv0199::Tn′blaTEM-1 mutant carrying a multicopy complementing vector expressing rv0199 from a constitutive promoter (pJES178). Error bars indicate SD of the means. *, P ≤ 0.05. The graph shows representative data from one of at least three independent replicate experiments.