FIG. 4.
M. tuberculosis ctaC::Tn′blaTEM-1 has a growth defect in macrophages and a colony phenotype on agar plates. (A) Relative location of ctaC in the M. tuberculosis genome. The black triangle indicates the site of Tn′blaTEM-1 insertion. (B) Membrane topology of CtaC predicted by the TMHMM algorithm (49). The site of ′BlaTEM-1 fusion is indicated with a triangle. (C) Macrophage infection as described in the legend for Fig. 3. Error bars indicate SD of the means. *, P ≤ 0.05. The graph shows representative data from one of at least three independent replicate experiments. (D) M. tuberculosis MBTB126 and the ctaC::Tn′blaTEM-1 mutant were plated on 7H10 agar plates supplemented with Tween 80. Colonies were examined after 25 days at 37°C.