TABLE 2.
Description of M. tuberculosis Tn′blaTEM-1 transposon library
| Parameter | Value |
|---|---|
| Total no. of transposon mutantsa | 81,000 |
| No. of confirmed carbenicillin-resistant insertion mutants | 177 |
| No. of in-frame insertions in ORFs | 172 |
| No. of unique ORFs identified | 111 |
| No. of ORFs with standard Sec signal sequence | 24 |
| No. of ORFs with lipoprotein-type Sec signal sequence | 15 |
| No. of ORFs with Tat signal sequence | 3 |
| No. of ORFs with transmembrane domainsb | 69 |
| No. of ORFs with homologues only in mycobacteria | 41 |
| No. of ORFs with homologues only in pathogenic | |
| mycobacteriac | 16 |
| No. of ORFs with no known or predicted functiond | 66 |
a
Estimated by plating a fraction of each transposition reaction mix on agar containing hygromycin only.
b
Includes proteins with at least one predicted transmembrane domain. Proteins with transmembrane domains plus a Sec, Tat, or lipoprotein-type signal sequence are most likely integral membrane proteins, and they are counted in this category.
c
Pathogenic mycobacteria searched included M. leprae, M. bovis, M. marinum, M. ulcerans, and M. avium.
d
Functions of individual ORFs are provided in Table S1 in the supplemental material.