TABLE 2.
Primer sequences
| Use | Primer | Sequence |
|---|---|---|
| DNA contamination | rplU-for | CGCAGTGATTGTTACCGGTG |
| rplu-rev | AGGCCTGAATGCCGGTGATC | |
| RT-PCR | 57RT′+ | CGGCGGTCGGCGATTCCCTC |
| 58RT′− | CAGCGAGCGGCGGCAGTAATC | |
| 58RT+ | CCTGGCCGAAGCCTCGCAACG | |
| 59RT− | GCGAATCCTCGGCATTCACCAG | |
| 59RT+ | GGAGCGCTATCGCTGCTG | |
| 60RT− | GTGTCGAGGCCGACGTGCAGC | |
| 60RT+ | GCTGCGACTGATCGCCCAGAC | |
| 61RT− | GCACCGAGATCATCACCAGG | |
| EMSA probes | 57promF-XbaI | GCTCTAGAGAGAGGTTCCGATGCGGATTG |
| 57promR-EcoRI | GGAATTCCGCAAGACCAGCAACAACTAC | |
| PA3757intF | GCTGCCCTCCGAACGCAACC | |
| PA3757intR | CCGTCCAGGTATTCGTAGAGG | |
| nagA flanking region | 57F-P1 | GGAATTCGAAGACAGCCCACGACCTGC |
| 57R-P1 | CGGGTGACTTTGCTCAGGTGCCGTGTCGGTTCAGCGTCGCAG | |
| glmS flanking region | 60F-P2 | CGTGTCGGTTCAGCGTCGCAGGCACCTGAGCAAAGTCACCCG |
| 60R-P2 | GGGGTACCCGCCGAGGGCCGCCTCGCTGTC | |
| Plasmid constructs | 61-FcompEcoRI | GGAATTCGCAACACTGGCCGCTGGCCTG |
| 61-RcompHindIII | CCCAAGCTTGTAGGGCGGATAACCGCTTGG | |
| 57promF-XbaI | GCTCTAGAGAGAGGTTCCGATGCGGATTG | |
| 57promR-EcoRI | GGAATTCCGCAAGACCAGCAACAACTAC | |
| NdeIPA14_15830F | GGAATTCCATATGAAGACAGCCCACG | |
| 3′N-57NdeI | GGAATTCCATATGTCAGCGTCGCAGT |