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High diacetyl formation with N-terminal truncated Ilv2. Beer fermentations were performed in MEX medium at 30°C with RKY2139 (WT, ▴) and RKY2491 (Ilv2Δ55, ○) for the times indicated. The sugar profiles and ethanol formation were similar to Fig. 1 (not shown). Experiments were performed at least three times. The results of a representative experiment are shown.
